Example 1: An experiment was conducted to study the hybrid seed production of bottle gourd (Lagenaria siceraria (Mol) Standl) Cv. Pusa hybrid-3 under open field conditions during Kharif-2005 at Indian Agricultural Research Institute, New Delhi. The main aim of the investigation was to compare natural pollination {the male flowers pinched from the seed parent before the anthesis regularly with utmost care to avoid the chance selfing. The pollination is carried out by the natural pollinating agent} and hand pollination {the male flowers also pinched from the seed parent before the anthesis regularly. The female buds are covered with butter paper bag which contain 5-6 tiny hole to felicitate the ventilation and to avoid the built up of high temperature in size the butter paper bag. The butter paper bag is clipped/ stippled. On the same day the male bud at pollen parent (male plant) are also covered with butter paper bag. On the next day the male bud are removed and the anthers are rubbed gently over the all three lobes. The female flower is again covered with butter paper bag and label is placed over the peduncle of pollinated female flower (plate 4, 5, 6 &7). The pollination is performed at noon (1-3pm)} under field conditions. The data were collected on 10 randomly selected plants from each of natural pollination and hand pollination. The data were collected on number of fruit set for the period of 45 days, fruit weight (kg), seed yield per plant (g) and seedling length (cm). The data obtained is as given below:

MS-EXCEL DATA FILE

  {Here 1 denotes natural pollination and 2 denotes the hand pollination}

1. Test whether the mean of the population of Seed yield/plant (g) is 200 or not.
2. Test whether the natural pollination and hand pollination under open field conditions are equally effective or are significantly different.
3. Test whether hand pollination is better alternative in comparison to natural pollination.

(The data received from Dr. B. S. Tomar, Division of Seed Science and Technology, IARI, New Delhi is duly acknowledged.)

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